به قشر انتورينال بر تشنج هاي ناشي از كيندلينك الكتريكي قشر پيريفورم در موش صحرايي A1 تاثير تزريق آگونيست اختصاصي گيرنده هاي آدنوزيني

The Effect of Intraentorhinal Injection of Selective Al Receptor Agonist on Piriform Cortex Kindled Seizures in Rats

Objective: In this research the role of adenosine At receptors of the entorhinal cortex on piriform cortex- kindled seizures was investigated. Materials and Methods: Animals were kindled by daily electrical stimulation of the piriform cortex. Then N6-Cyclohexyladenosine (CHA), an adenosine A, receptor agonise, at concentrations of 1,10 and 100 miuM and I,3-dimethyl-8 cyclopenthylxanthine (CPT), an adenosine Al receptor antagonist, at concentrations of 50 and 100 miuM were injected into the entorhinal cortex. Animals were stimulated 5 min after drug injection and seizure parameters were recorded. Also in another group of animals, CPT (50 miuM) was injected 5 min before CIIA (100 miuM). Results: CIIA at a concentration of 100 miuM decreased the afterdischarge duration (ADD), stage 5 duration (S5D), and seizure duration (SD), and increased the latency to stage 4 of the seizure (S4L) significantly. CHA at a concentration of 10 miuM decreased only SD and increased S41_ significantly. On the other hand bilateral microinjection of CPT into the entorhinal cortex at a concentration of 100 µM increased ADD, S5D, and SD, and reduced S4L significantly. Pretreatment of animals with CPT (50 miuM), 5 min before CHA (100 miuM), reduced the effect of CHA on seizure parameters. Conclusion: These results suggest that adenosine A, receptors activity in the entorhinal cortex reduces piriform cortex kindled seizures.