بررسي ارتباط پلي مورفيسم ژن TNFR1 با ناباروري ايديوپاتيك مردان

Association of TNFR1 gene polymorphism and idiopathic male infertility

هدف: در مطالعه حاضر، ارتباط پلي مورفيسم TNFR1 36A/G با ناباروري ايديوپاتيك مردان در جمعيت گيلان بررسي شد. مواد و روش‏ها: اين مطالعه شامل 106 مرد نابارور ايديوپاتيك و 114 مرد بارور به‏عنوان گروه كنترل مي باشد. نمونه خون تهيه و DNA ژنومي استخراج شد. سپس ژنوتيپ‏ها و فراواني آللي با استفاده از روش PCRRFLP ارزيابي و آناليز آماري با استفاده از نرم افزار  MedCalc  انجام شد. نتايج: فراواني ژنوتيپ‏هاي AA, AG, GG بيماران به‏ترتيب برابر 9/50، 5/7 و 5/41 درصد و نمونه‏هاي كنترل به‏ترتيب برابر 1/21، 3/33 و 6/45 درصد بود. فراواني نسبي آلل A و G بيماران به‏ترتيب برابر 45/0 و 55/0 بود و نمونه‏هاي كنترل به‏ترتيب برابر 62/0 و 38/0 بود. نتايج نشان داد در فراواني ژنوتيپي(P=0.002)  و آللي (P=0.0004) گروه‏هاي نابارور و كنترل ارتباط معني‏دار ديده مي‏شود.  نتيجه گيري: در نتيجه، به‏نظر مي‏رسد در استان گيلان افراد با آلل G و ژنوتيپ GG بيشتر در معرض خطر ابتلا به ناباروري ايديوپاتيك مي‏باشند. مطالعات ديگري با تعداد بيشتري از بيماران جهت نشان دادن نقش پلي مورفيسم ژن TNFR1 در ناباروري مردان مورد نياز است.

Aim: Infertility is the failure of a couple to engender after endeavoring at least one year of unprotected intercourse. male factor infertility accounts for approximately 50 percent of causes. Tumor necrosis factorα (TNFα) is a multifunctional cytokine. TNFα plays important role in the regulation of cellular processes related to spermatogenesis. There are two variants of the cell receptors that interacts with TNFα. In the present study, the association of TNFR1 36A/G polymorphisms with idiopathic male infertility in the Guilan population was studied. Materials and Methods: This study consists of 106 infertile men and 114 fertile men as control group. Blood samples were taken and genomic DNA was extracted. Then genotypes and allele frequencies were assessed by PCRRFLP method and the statistical analysis was performed by MedCalc software. Results: The frequencies of AA, AG, GG genotypes in patients were 41.5%, 7.5% and 50.9%, respectively and in controls were 45.6%, 33.3%, and 21.1%, respectively. The frequencies of A and G in patients were 0.45 and 0.55, respectively and in controls were 0.62 and 0.38, respectively. The results showed that there is a significant association between genotype frequency (p= 0.002) and allele frequency (p= 0.0004) in infertile and control groups. Conclusion: In conclusion, the subjects with G allele and GG genotype appears to be at greater risk of developing idiopathic infertility in Guilan province. Further studies with larger numbers of patients are required to elucidate the potential role of TNFR1 polymorphism in male infertility.