در حيوان آزمايشگاهي CORIANDRUM SATIVUM L بررسي آثار ضدالتهابي و ضددارويي گياه گشنيز

Anti-inflammatory and analgesic effects of Coriandrum sativum L. in animal models

An important. part of ethnopharmacology is study of pharmacological effects of medicinal plants and natural compounds m animal models. ln this study the anti-inflammatory and analgesic effects of Coriandrum sativum L. seeds were evaluated. The total hydroalcoholic and flavonoid extract of the seeds were prepared and concentrated. Essential oil of coriander was obtained by soxhlet extractor, using pentane as solvent. Carrageenan (CAR) test was used for evaluation of anti-inflammatory effect. ln this test 100ml of CAR suspension (1 %w/v) was injected into subplantar space of rat hind paw and volume of paw was measured at 0 and 3 hr, after injection. For evaluation of analgesic effect writhing and formalin tests were used. For writhing test, acetic acid (l%v/v) at a dose of I0 ml/kg was injected intraperitonealy (i.p.) and 10 min later the number of writhes was counted during a 0 minutes period. ln formalin test, the paw licking time was measured during phase 1(0-5 min) and phase 2 (20-25min) after injection of 50 pi of formal in (2.5%) iv). Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) analysis showed that I linalool (83.1%) is the major constituent of coriander essential oil. Our results showed that Coriander had no anti-inflammatory effect in CAR test. in writhing test. only the essential oil (4m1/100g.P.O) had a significant effect (P<0.01).