جذب سرب توسط باكتري سودوموناس پوتيدا (p168)و كمپوزيت هاي اين باكتري با رس هاي پاليگورسكيت و سپيوليت از محلول هاي آبي

Lead Sorption from Aqueous Solutions by Pseudomonas putida (p168) and its Composites with Palygorskite and Sepiolite Clays

امروزه آلودگی محیط زیست به انواع فلزات سنگین در اثر توسعه صنایع به یك تهدید جدی تبدیل شده است. سرب از جمله این فلزات است كه اثرات نابهنجاری بر محیط زیست و سلامتی موجودات دارد. هزینه زیاد و مشكلات تكنیكی روش های معمول حذف فلزات سنگین باعث شده روش حذف بیولوژیكی بوسیله باكتری ها به عنوان گزینه ای اقتصادی و سازگار با محیط زیست مورد توجه قرار گیرد. برای انجام این آزمایش، باكتری ها در محیط كشت NB كشت شدند و با استفاده از روش چگالی نوری و میكروسكوپ نوری جمعیت آن ها تعیین گردید. میزان جذب سرب تهیه شده از محلول نیترات سرب توسط بیومس خشك باكتری اندازه گیری شد. برای تعیین همدمای جذب سرب توسط كمپوزیت های رس- باكتری، نسبت های مختلف باكتری- رس با استفاده از نیترات پتاسیم 01/0 مولار تهیه و با محلول 5/0 میلی مولار سرب مخلوط گردید. نتایج جذب سرب در غلظت های 5/7 تا 5/55 میلی گرم بر لیتر توسط باكتری سودوموناس نشان داد كه حدود 5/31 تا 4/78 درصد از غلظت های اولیه سرب توسط این باكتری جذب می شود. حداكثر ظرفیت جذب سرب (qmax) توسط سودوموناس پوتیدا 4/582 میلی گرم بر گرم به دست آمد. بر اساس آزمون LSD تمامی نسبت های مختلف كمپوزیت ها در سطح احتمال آماری 001/0 تفاوت معنی داری با باكتری منفرد در جذب سرب داشتند. نتایج نشان داد كه باكتری سودوموناس پوتیدا و كمپوزیت های این باكتری با رس های پالیگورسكیت و سپیولیت پتانسیل زیادی برای حذف سرب از محلول های آبی دارند.

Introduction: Heavy metals contamination due to natural and anthropogenic sources is a global environmental concern. Lead (Pb) is one of the very toxic heavy metals. Industrial production processes and their emissions, mining operation, smelting, combustion sources and solid waste incinerators are the primary sources of lead. This heavy metal has aberrant effects on the environment and living organisms. Hence, proper treatment of lead from soil and industrial wastewaters is very important. In order to remove toxic heavy metals from contaminated water systems, conventional methods such as chemical precipitation, coagulation, ion exchange, solvent extraction and filtration, evaporation and membrane methods are being used. These conventional methods generally have high costs and technical problems. Therefore, biosorption processes, in which microorganisms are used as sorbents, have been considered as economical and environmentally friendly options for removal of heavy metals from aqueous solution. Clay minerals are another group of sorbents used in removal of heavy metals from polluted environments. Furthermore, bacterial cells can be attached on clay mineral surfaces and form bacteria-mineral composites. These composites adsorb heavy metals and convert them into forms with low mobility and bioavailability. Pseudomonas putida is a unique microorganism with a high tendency to sorb and/or degrade certain environmental pollutants. Palygorskite and sepiolite are the fibrous clay minerals of arid and semiarid regions; their structures consist of ribbons and channels. These fibrous minerals have various applications in industry and the environment because of its large surface area and high adsorption capacity. The present study was conducted in order to determine the ability of Pseudomonas putida (P168), and its composites with palygorskite and sepiolite in lead sorption. Materials and Methods: The bacterial strain used in the present study was Pseudomonas putida (P168) grown and maintained on Nutrient Broth (NB). The population of living and non-viable bacteria in suspension was determined by an optical microscope. The minerals used in this study were palygorskite from Florida (the Source Clay Minerals Repository, Purdue University, IN) and sepiolite from Yazd (Iran). The clay samples were ground and passed through 0.05 mm (mesh #270) sieve. The clays were then saturated with calcium chloride (0.5 M) and washed free of salts. Batch experiments were performed to measure Pb sorption by Pseudomonas putida. For this purpose, 10-ml aliquots of bacterial suspension (7.24×107 cells ml-1) were added to10 ml solutions containing Pb with concentration ranged from15-110 mg L-1. The mixtures were gently shaken at 30 ◦C for 24 h and centrifuged at 3000 rpm for 20 min. The concentration of Pb in the supernatants was finally measured by atomic absorption spectrometer. The percentage of sorbed Pb was determined by subtracting the amount of unabsorbed Pb from that initially added. Various hybrids of P. putida and clays were also exposed to solution of 0.5 mM Pb in 0.01 M KNO3 to determine the role of composites in sorption of Pb. Langmuir and Freundlich adsorption isotherms were chosen to describe the biosorption equilibrium data. GraphPad Prism 5.0 was used for determining the isothermal parameters using non-linear regression analysis. Data were analyzed with the Statistical Analysis System (SAS). Experimental design was factorial in form of complete randomized block. Results and Discussion: Pseudomonas putida showed a considerable capacity to sorb Pb ions. Lead sorption isotherms were sufficiently fitted with the Langmuir and Freundlich models. The Pb sorption isotherms by P. putida were L-type showing a high affinity of P. putida for Pb ions. Lead sorption capacity (qmax) of P. putida was estimated to be 582.4 mg g-1 and its Langmuir constant (KL) was found to be 0.11 mg L-1. The experimental data of lead sorption (7.5-55.5 mg L-1 initial concentration) by P.putida (P168) demonstrated that about 31.5% to 78.4% of the intial concentration of Pb was taken up by these bacteria. Sorption of Pb decreased with the increase of bacteria in the bacteria-clay composites, which may be due to the occupation of adsorption sites on the clay surface by the bacteria. Composites of bacteria-sepiolite were more effective than bacteria-palygorskite in Pb sorption due to the larger channel dimensions, greater surface area, and more functional groups of sepiolite than palygorskite. LSD test showed that there were significant differences between the hybrid sorbents with different ratios and single bacterial cells in Pb sorption. Conclusion: The results showed that P. putida and its composites with palygorskite and sepiolite clays exhibited a high potential for the removal of Pb from aqueous solutions.