Bald/c مطالعه توزيع برخي گليكوكانژوگيتها در نوتوكورد و عروق محوري در اوايل دوران مورفوژنز در جنينهاي موش

Distibution of some glycoconjugates in notochord and axial vesseles during early morphogenesis in Balb/C mouse embryos

Background and Objectives: Lectins are carbohydrate-binding proteins of plants or animal origin, which react with terminal sugars of glycoconjugates of cells and tissues. Glycoconjugates play essential role in several biological phenomena shuch as; cell recognition, proliferation, migration and differentiation. Notochord, a mesodermal drivative, induces nearby tissues, including; neural tube, somites and developing gut. Materials and Methods: The purpose of this study was to investigate distribution of glycoconjugates in component of notochord and axial vessels during mouse embryonic development. Results: Formaline fixed sections embedded in paraffin from 9 to 14 embryonic days of balb/c mice were processed for histochemical studies by using four different horseradish peroxidase (HRP) labelled lectins including; Glycin max (SBA), Vicia Villosa (VVA) and Maclura pomifera (MPA) specific for GalNac terminal sugar and Lotus tetragonolobus (LTA) that bind to a-L-fucose terminal sugar. Our results revealed that approximately similar reactions were observed in notochord and axial vessels endothelium by using GalNac sensitive lectins during vessels morphogenesis. Fucose sensitive lectin reacted only with the notochord. The time and significant role (s) in tissue interactions and subsequent formation of adjacent tissues such as; axial vessels development during critical morphogenic period. Conclusion: Furthermore, our findings indicate that fucosylated glycoconjugates may have no role in axial vessel formation and / or their interactions with the notochord during early morphogenic period.