طراحي روش ايمونوفلورسانس غيرمستقيم براي سنجش پادتن هاي ضد سپتوپلاسم نوتروفيل

ANCA are classes of Antibodies or in reality Autoantibodies usually produce in conditions that degradation of neutrophiles occur. This is led to vascular damage. They are more common in Rheumatic diseases such as Rheumatoid Arthritis (RA), systemic lupus Erythematus (SLE) and vasculitic diseases especially Wegnersʹ granulomatosic (with specificity and sensitivity as high as 95%). We decided to design IFA-ANCA because this assay has high sensitivity and in with comparision other techniques such as ELISA & RL4 is more economic and simple. On the other hand , screening test ,for determining ANCA is immunofluorescent assay. ANCA test establishment contains : Isolation of neutrophiles , fixation with ethanol and ,formalin , improvement of method and quality control by using of indirect immuno flourescence assay (IFA), two staining pattern are observed. Cytoplasmic (C-ANCA) or classic and prinuclear (P-ANCA) pattern. Established ANCA test by inter assay method had 95% reproducibility and 91% precision.