بررسي سرمي و رديابي كوكسيلا بورنتي در گاوهاي شيري اهواز به روش الايزا و واكنش زنجيره اي پلي مراز

Serological Survey and Detection of Coxiella burnetii in Dairy Cattle of Ahvaz by ELISA and PCR

تب كيو يك بيماري زئونوز و عامل آن كوكسيلا بورنتي است. حيوانات اهلي مهمترين مخزن كوكسيلا هستند و از طريق شير، ادرار، مدفوع و ترشحات توليد مثلي آن را دفع مي­كنند. آلودگي انسان و حيوانات عمدتاً از طريق استنشاق آئروسل­ها مي­باشد. هدف از مطالعه حاضر تعيين شيوع كوكسيلا بورنتي در شير و سرم گاوهاي شيري ارجاعي به بيمارستان دانشكده دامپزشكي دانشگاه شهيد چمران و همچنين ارتباط آن با تعيين كننده­هاي ميزباني و مديريتي مي­باشد. در اين تحقيق نمونه­هاي خون و شير 86 رأس گاو شيري جمع آوري گرديد و سرم­هاي تهيه شده به روش الايزا از نظر حضور آنتي­بادي عليه كوكسيلا بورنتي و نمونه­هاي شير به روش واكنش زنجيره­اي پلي­مراز از نظر حضور كوكسيلا بورنتي آزمايش شدند. شيوع كوكسيلا بورنتي در شير و سرم به ترتيب 65/4% و 49/3% بود. رگرسيون لاجستيك تك متغيره نشان داد شانس آلودگي(نسبت شانس 17/1 و فاصله اطمينان 95% 52/1- 89/0) با افزايش سن افزايش مي­يابد (05/0

Q fever is a widespread zoonosis caused by Coxiella burnetii. Domestic animals are the most important reservoirs of C. burnetii, which is exerted in the milk, urine, feces and reproductive discharge of infected animals. Inhalation of bacteria is the main route of animal and human infection. The aim of this study was to survey prevalence of C. burnetii in serum and milk of referred cows to veterinary hospital of Shahid Chamran university and correlation of this organism with host and management determinants. Milk and serum samples from 86 cows were collected and were examined by PCR and ELISA assay, respectively. Prevalence of Q fever in milk and serum samples was 4.65% and 3.49%, respectively. Logistic regression showed that the odds of infection was increased with increase of age (OR: 1.17 and 95% CI: 0.89-1.52, P>0.05) and 3.4% of fluctuation of infection was justified by age. Odds of infection in industrial husbandry than traditional was 2.81(95% CI: 0.53-15.02, P>0.05) and 4.1% of fluctuation of infection was justified by husbandry. 3.8% of fluctuation of infection was justified by fertility status and odds of infection in cows with infertility than healthy was 3.16 (95% CI: 0.35-28.37, P>0.05). The result showed excretion of C. burnetii in milk and the fact that bacteria is in correlation with infertility in cows. Thus efforts must be taken to control and prevent Q- fever.