توليد ريزغده هاي سالم سيب زميني در سيستم بيوراكتور تناوبي

Production of healthy microtubers of potato in after native bioveactor system

The present investigation was carried out to determine efficiency of in vitro bioreactor-based tuberization of potato. In vitro potato stock plants (Solanum tuberosum L. CV. Agria) were obtained from apical meristems followed by thermotherapy. These in vitro plants were multiplicated by serial subcultures in 300 ml jars containing MS liquid medium. Cultures were incubated under 24/22 °C day/night temperature regime with a 16-h photoperiod fluorescent light at an irradiance of 3000 lux (36 pmol m-2 sʹ). Multiplication medium consisted of MS Salts and Vitamins, 30 gL-l Sucrose, 2 mgL-ʹ 6-Benzylaminopurine (BAP), 0.1 mgLʹʹ Naphthalene Acetic Acid (NAA) and 1.0 mgL"ʹ Gibberellic Acid (GA3). Tuber induction medium was the same as basal medium except 80 g sucrose, and 7 mg Lʹʹ BAP were used. Shoots produced in a 300ml jar were transplanted into a 3L bioreactor vessel and the medium was pumped into it every 8 hrs. to immerse the shoots for about 30 minutes. The entire medium was changed with tuber induction liquid medium after 4 weeks. These cultures were incubated under continuous dark condition at 20 °C for 4 weeks. The growing shoots were subcultured from 300mi jar to bioreactor in which they continue to grow. After 3 days tuber induction was observed. The tuberization occurred mainly at middle part of the bioreactoʹr. The total number of microtubers in this 3L bioreactor was about 550 and was very more efficient than routine system (3 to 4 microtubers per 300 ml jar).