بررسي تاثير روشهاي تلقيح مصنوعي سرويكال و رحمي برنتايج آبستني ميش هاي شال درخارج از فصل توليد مثل

Comparison of methods of cervical and uterine artificial insemination of Shall ewes on pregnancy rate in non-breeding season

Forty Shall breed of 2-4 years old ewes were selected in Research Centre of Faculty or Veterinary, University of Tehran. In spring, estrus synchronization of ewes were carried out using CIDR containing progestrone hormone for a period of 12 days with an intramuscularly injection or PMS(i alter its removal from vagina. Semen samples was collected from five Shall breed of rams using an artificial vagina. Suitable semen samples were pooled and diluted with sterilized and homogenized cow milk at 30ʹ4 . Straws were filled with 0.2 ml of diluted semen and stored at 18ʹ4 for 4 hours until use. Concentration of diluted semen was measured by haemocytornetry method. Estrus synchronized ewes detected by a teasere ram and divided into two groups For cervical and uterine insemination. Alter 50 hours or PMS6 injection, artificial insemination was carried out by placing a vaginoscope into vagina, fixing the cervix by a forceps, forcing a bented-tip pipette, specially designed to pass the cervical folds through cervical lumen. Pipette was pushed forward by gentle rotation as far as possible and semen was injected either in cervical folds or in uterine body. Non-return and pregnancy rates of ewes were measured after 16-17 and 30 days ofʹ artificial insemination. All ewes were synchronized and non-return rates of inseminated ewas was 100%. Mean pregnancy rate of inseminated ewas was 47.5%. for young and aged ewas 52 and 43% respectively. Mean pregnancy percentages of ewas inseminated cervically were 42.5 and 52.5 for uterine insemination. The Results showed that effect of place of injection of semen on ewas pregnancy rates was not significant (P>0.05).