ORYZZA SATURAL مطالعه كالوس زايي و باززايي گياه از طريق كشت جنين رسيده در ارقام مختلف برنج

Investigation of callus induction and plant regeneration through tissue culture in Iranian rice cultivars

This research was performed in order to evaluate five Iranian rice cultivars (cvs. Sange-Tarum, Mahalli- Tarum, Deilamani- Tarum, Hassani and Hassan saraie) for studying the percent of callus induction and plant regeneration on two culture medium of Murashige and Skoog (MS) and Linesmair and Skoog (LS). After dehusking and sterilization, seeds were cultured on the callus induction medium MS and LS including 2. 2.5 and 3 mg/l 2, 4-D. Then , petri dishes were incubated at 26±1 - The percent of callus induction and embryogenic callus were compared in different varieties. Then, the embryogenic calli were transferred on LS and MS medium, with different proportion of hormones such as kinetin(l, 2 . 3 mg/l) , BAP (1, 2. 3 mg-/1) and NAA (05, 1, 2 mg/l). The callus incubated in the dark or under fluorescent light at 26±1ʹc for plant regeneration. Results showed that the first group included two cultivars of Deilamani Tarum and Hassan saraie with average of callus induction 80 and 78.67 percent representatively, second group included two genotypes of Mahalli Tarum and Hassani with average of callus inductuin 70.33 and 73.67 percent, representatively and third group included Sang-e- Tarum that its average callus induction was 66.33 percent. There were many differences in regeneration between Iranian rices; cv. Deilamani Tarum had the maximum percent of regeneration between studied cultivars and cvs. Mahalli Tarum, Hassani, Sang e-Tarum and Hassan saraie placed after that. The results showed that LS medium was better for callus induction and plant regeneration in the different cultivates.