LC05 و تعيين )Acipenser Stellatus( اثر سم ديازينون روي شاخص هاي خوني ماهي خاوياري ازون برون

Effects of Diazinon on the hematological profiles of Acipenser stellatus and determination of LC50

On hundred eight specimens of juveniles of stellate sturgeon ,Acipenser stellatus with average body weight of 7±l gr in six groups (5 experimental &l control groups) were examined at Ecological Institute of Caspian sea. After determination of lethal concentration (3.7 mg/L) fish exposed to 3, 4, 5, 6 and 7 mg/L of diazinon.The LC50, 96 hr of diazinon for Acipenser stellatus calculated as 4.98 mg/L. Specimens showed abnormal behavior such as severe stress, spasm of muscels around the mouth, semicircular summing and disruption of breath activities.To study the hematological parameters,blood were taken from the fish and values of RBC,PCV, Hb, MCV, MCH and MCHC in experimental and control groups showed significant differences (P<0.05). Results from differential counts of leucocytes and lymphocytes of the groups showed significant differences (P<0.05).In the biochemical values of total protein and glucose of blood serum of examined groups showed significant differences (P<0.05). During the study, DO, PH, water hardness, N03, N02, EC, P04 and water temperature were also recorded daily.