CITRUS PARADISII MACF بررسي ميزان فلاونوييد نارنجين در مراحل مختلف نمو ميوه گريپ فروت مارش

Investigation of the naringin flavonoid content during different fruit development of marsh grapefruit(Citrus paradisii Macf.)

The experimental design was conducted at the Citrus Research Institute, Kotra, Tonekabon located in the north of Iran .We have used split-plot design on the basis of randomized completely in three replications. In this investigation the effects of fruit harvest time of Marsh grapefruit (Citrus paradisii Macf.) on weight, diameter, peel thickness, dry matter, total extract and naringin content in the total extract were evaluated. Extraction of total extract took place by hexan and measurement of naringin used by high performance liquid choromatography (HPLC). On the basis of the results, fruit harvest time had significant effect on the mentioned parameters. Fruit diameter variation was proportional to the fruit weight and was pretty rapid. Fruit peel thickness at the first step of growth increased (8/3mm) then with fruit development and growth it decreased (3/8mm). Precentage of dry matter at the first step of harvest increased (35/94%) then decreased (30164%). The total extract significantly affected by fruit harvest time. The highest total extract (3 g/100g DW) obtained from the fruits were harvested 65 days after full bloom. Results also show that the highest naringin content (61.59% of total extract) found in the fruit was obtained 45 days after full bloom.