الگوهاي تفكيك ژن هاي اصلي شناخته شده گلوتنين هاي داراي وزن ملكولي بالا و پايين با استفاده از هاپلوييد مضاعف حاصل از تلاقي بين گونه اي گندم و ذرت

Segregation patterns of known major genes in the doubled haploid population derived from (Trident * Molineux) wheat F1 * maize crosses I. determination of endosperm protein phenotypes

To utilize doubled haploid (DH) lines for plant breeding purposes, it is desirable that a random sample of parental gametes is obtained because it is possible to predict progenies. In current study 256 DH lines derived from intervener hybridization have been undertaken between wheat and maize using F 1 hybrids of wheat cultivars. Trident and Molineux have been used as parental cultivars of wheat F1 hybrids in 1997 at Waite Campus. Gliadin and Glutenin were extracted from each DH lines according to a method developed by Sing et al. (1991) and to separate glutenin subunit bands, a Hoefer gel electrophoretic apparatus has also been used. The current study of glutenin subunits related loci gave no case of distorted segregation patterns showing departure from expected Mendelian ratios. This case shows that DH progeny of present work can be used in breeding programs for improving bread quality characteristics of wheat and doing selection for the benefiting of high quality cultivars.