شناسايي ، تعيين پراكنش و خالص سازي ويروس هاي موزاييك هندوانه تيپ 1 و 2 در مناطق عمده كشت كدو در استان خراسان

Identification, determining distribution and purification of Watermelon mosaic virus-1 and Watermelon mosaic virus-2 in Zucchini squash fields of Khorasan province

Among different diseases of zucchini squash, virus diseases cause severe damage in this crop every year. Viruses in the potyvirus genus (Potyviridae family) are the major viruses infecting zucchini squash. In the spring and summer of 2001, successive sampling accomplished from zucchini plants with symptoms such as mosaic, malformation and color breadking of leaf and fruit blistering at the different stages of growth in Khorasan province (in the main growing areas of zucchini squash). Detection of viruses was based on ELISA tests. Identified potyviruses were watermelon mosaic virus- ( WMV-1) and watermelon mosaic virus-2 (WMV-2). For determining distribution of these viruses, samples were collected randomly in the fields and were tested by DAS-ELISA. The infected samples with WMV-1 and WMV-2 were 98 and 134 out of 500 collected samples respectively. Some of the samples show infection with these two viruses simultaneously, this shows that viruses do not crossprotect each other. Host range of WMV-2 was larger than WMV-1. Watermelon mosaic virus-2 (WMV-2) was purified. The purification was confirmed by spectrophotometry and coat protein electrophoresis. The molecular weight of WMV-2 coat protein was 34047 dalton.