استقرار درون شيشه اي سه رقم زيتون

In Vitro establishment of three olive cultivars (Oleo europaea L.)

Tissue culture systems provide a high efficiency for mass propagation of desired disease-free plants as well as genetic improvement. Considering these facts and the importance of olive crop, some experiments were carried out to investigate the in vitro establishment of three olive cultivars. For this purpose, single nodes were taken from mature container grown ʹManzanillaʹ, `Dezfoulʹ and `Lardʹ olive cultivars. to Lind out the best disinfection method, different concentrations of NaOCI and HgCI, were used. Three sampling times i.e. 4ʹ1ʹ of February, 51h of May and 6ʹ1ʹ of August and three culture media i.e. OM, MS and MS with half strength of macro elements (1I2MS) were compared to optimize shoot establishment. The most important contamination agent was diagnosed and the controlling effect of Celotaxirne antibiotic was evaluated. Furthermore, the effects of two cytokines: Benzyladenine (BA) and Zeatin on establishment were evaluated. The Results showed that treated explants with 0.1% HgCI, for 3 min. followed by three times rinsing with sterile distilled water was the most appropriate disinfection method. Explants, which were taken on 6th of August and grown in 112 MS medium, gave the best results. The main contaminating agent was Rseau.lomona.s bacterium which was controlled by Cefotaxime at 100 mg/I. adding 0.5 mg/I BA to 112 MS medium improved in vitro establishment of olive explants.