پديد آورندگان :
عظيم زاده زهرا نويسنده , چمنی اسماعیل نويسنده استاد گروه علوم باغبانی، دانشكده كشاورزی، دانشگاه محقق اردبیلی Chamani Esmail , عرفانی ملیحه نويسنده دانشجوی دكتری، گروه علوم باغبانی، دانشكده كشاورزی، دانشگاه محقق اردبیلی Erfani Malihe
كليدواژه :
بنزيل‌آدنين , درون‌شيشه‌اي , ريشه زايي , نفتالين‌استيك اسيد
چكيده فارسي :
سوسن چلچراغ (L. ledebourii) كمیاب‌ترین گونه‌ی جنس سوسن است كه بیشتر در ناحیه‌ی قفقاز می‌روید. ایران نیز یكی از مهمترین مناطق پراكنش این گونه در حال انقراض است. لزوم توجه به حفظ این گیاه و استفاده از آن در كارهای اصلاحی و انتقال ژن، نیاز به باززایی فراوان آن در شرایط درون شیشه‌ای دارد. بدین منظور در این تحقیق سعی شد تا با استفاده از تنظیم كننده‌های رشد گیاهی و یك تنش غیرزیستی (فراصوت) توانایی پیازچه‌زایی و ریشه‌زایی این گیاه مورد بررسی قرار گیرد. این پژوهش به صورت آزمایش فاكتوریل در قالب طرح كاملاً تصادفی در چهار تكرار صورت گرفت. نتایج حاصل از پژوهش نشان داد كه تنظیم‌كننده‌ی رشد NAA نقش مهمی در افزایش وزن كل پیازچه‌های تولید شده دارد، به‌طوری‌كه بیشترین مقادیر این صفت از غلظت 1/0 و 1 میلی گرم در لیتر NAA حاصل شد. همچنین بیشترین تعداد ریشه در هر پیازچه از تركیب تیماری 01/0 میلی گرم در لیتر BA بدست آمد. در تركیب غلظت‌های مختلف هورمون NAA و هورمون BA، بیشترین وزن كل پیازچه‌ها در ریزنمونه‌ی پیازچه در محیط كشت MS حاوی 1/0 میلی گرم در لیتر NAA بدون BA بدست آمد. همچنین در غلظت بالای BA نسبت به NAA، همه‌ی سطوح فراصوت باعث افزایش تعداد فلس‌ها و وزن پیازچه‌ها گردید و بیشترین تعداد پیازچه‌ها از 10، 20 و 30 ثانیه فراصوت‌دهی حاصل شد. اثر مثبت تیمار فراصوت بر پیازچه‌زایی و صفات مربوط به آن نشان می‌دهد كه این تیمار می‌تواند تاثیر زیادی برای باززایی این گیاه در شرایط درون شیشه‌ای داشته باشد. بنابراین از آنجا كه فراصوت به طور غیرمستقیم بر میزان هورمون‌های درون‌زا در ریزنمونه تاثیر می‌گذارد، توصیه می‌گردد كه در كنار تیمار هورمونی می‌توان از تیمار فراصوت در تكثیر این گیاه با ارزش استفاده كرد و با استفاده از آن میزان باززایی از این گیاه را افزایش داد.
چكيده لاتين :
Introduction: Lily (L. ledebourii) is the rarestspeciesof thegenusLilium, and grows in Caucasus region. Iranis one of the important distribution areas of this endangered species. It is important as an ornamental plant due to its large and attractive white flowers that are equal to those of commercial lilies in terms of beauty.The two main constraints on growing this plant are a low multiplication rate and the high cost of bulb production. Five to ten flowers commonly appear on each plant, even specimens with up to 15 flowers have been observed. Plant tissue culture techniques are widely used in plant propagation and using these methods can effectively provide micro-propagation of this plant in large scale. High percentage ofregeneration is necessary for plant protection, using in the breeding programs and gene transfer to this plant. Therefore, the effect of plant growth regulators and abiotic stress (ultrasound) werestudied on the bulblet production and root induction of Lilium ledebourii.
Materials and Methods: The experiment was factorial based on completely randomized design with four replicattions and was carried out in tissue culture lab of University of MohagheghArdabili in 2015. For this purpose, segmentsof scale explant was treated with ultrasound and cultured on MS medium supplemented with different concentrations of NAA and BA alone and/or in combination with each other. In this experiment, different concentrations of NAA (0, 0.01, 0.1 and 1 mgl-1) and BA (0, 0.01, 0.1 and 1 mgl-1) and different Ultrasound exposure duration (0, 5, 10, 20 and 30 second) were studied. In order to remove possible contamination from the media, all media were autoclaved for 20 minutes at 121 °C. At the end of the experiment, the number of bulblet, root length, fresh weight of bulblet were recorded. The cultures were kept at 25±2°C under illumination with daylight fluorescent lamps (30 mol m-2s-1) at 16 h photoperiod. Data was subjected for analysis of variance and compare means using SPSS 16.
Results and Discussion: The results showed that ultrasound had negative effect onroot length, so that the highest root length was observed in explants without ultrasound treatment. Result also indicated that ultrasound had positive effect on bulblet production and root induction. A different effect of growth regulators was observed in similar media on the bulblet formation percentage. The 0.1 NAA concentration had a higher efficiency while increasing NAA insignificantly decreased bulblet induction. The highest total weight and number of bulblets obtained by 0.1 mgl-1 NAA. Concentrations of NAA increased rooting percentage. Different concentrations of NAA had also significant effects on some traits. So that, the highest weight of bulblets obtained by 0.01 and 0.1 mgl-1 BA and the highest number of roots obtained in control. Bulblet maximum mean weightwas in30 seconds ofultrasoundtreatment, which hada significantdifference with the control treatment (without ultrasoundtreatment). In the other hand, ultrasound increased the number and weight of bulblets.Mechanical stress and microstreaming by acoustic cavitation might be considered as the most possible cause of the various physiological effects of ultrasound on cells. The enhancement of V-ATPase transport and ATP hydrolysis activities seem to be an ultrasound-induced metabolic response of cells. High-intensity ultrasound is well known to be destructive to biological materials, disrupting the cell membranes and deactivating biological molecules such as enzymes and DNA. Low-intensity ultrasound, on the other hand, has shown a range of sub lethal biological effects that are of potential significance in biotechnology. There are several processes that take place in the presence of cells or enzymes activated by ultrasonic waves. High-energy ultrasonic waves break the cells and denature the enzymes. Low-energy ultrasound can modify cellular metabolisms or facilitate the uptake of nutrient, and make them easily through the cellular walls and membranes. In the case of enzymes, the increase in the mass transfer rate of the reagents to the active site seems to be a most important factor.
Conclusions: The results showedthatthebulblet production at first stages and a little root formation in tissue culture is useful for fast bulblet inductionandthenrooting. Finally, it seems that ultrasound in combination with plant growth regulators have the potential to produce the highest average number of bulblets in the scale explant.
