بررسي ارتباط بين پلي مورفيسم D/I ژن آنزيم مبدل آنژيوتانسين با چاقي در جمعيت استان اردبيل

The Association Study between D/I Polymorphism of Angiotensin-Converting Enzyme (ACE) Gene with Obesity in the Population of Ardabil Province

سابقه وهدف: امروزه ژن هاي زيادي كه تاثيرهاي پيچيده اي بر چاقي دارند گزارش شده است و يكي از اين ژن ها آنزيم مبدل آنژيوتانسين (ACE) است كه عامل موثر در متابوليك بافت چربي مي باشد و در رشد و عملكرد سلول هاي آدپيوسيت درگير مي باشد. هدف از اين مطالعه، بررسي ارتباط بين اين پلي مورفيسم با چاقي در جمعيت استان اردبيل بود. مواد و روش ها: دراين بررسي 172 نفر بر اساس شاخص توده بدني به دو گروه افراد و افراد چاق با تقسيم شدند و DNA نمونه ها با استفاده از روش نمك اشباع استخراج شده و ژنوتيپ افراد تعيين گرديد. يافته ها: درگروه ، 5/57% مردو 5/42% زن وگروه 42% مردو 58% زن بودند. ميانگين سني افراد ،5/47 سال و ميانگين سني افراد 5/52 سال بود. ميانگين BMIافراد ، 19/25 و ميانگين BMI افراد ، 58/32 مي باشد. بحث: فراواني ژنوتيپDD در افراد چاق با ، 83/0 و در افراد 71/0بود كه اين اختلاف ازنظرآماري معني دارمي باشد (044/0 ). نتيجه گيري: يافته هاي حاصل از اين تحقيق نشان داد كه ژنوتيپ DD در ژن ACE مي تواند با چاقي جمعيت استان اردبيل ارتباط داشته باشد.

Aim and Background: Today many genes have been reported that affect obesity in complex ways. One of these genes is the polymorphism of ACE gene. Angiotensin converting enzyme (ACE) is an important factor that can affect adipose tissue metabolism. The aim of this study was to investigate the relationship between ACE gene polymorphism and obesity in the population of Ardabil province. Methods and materials: The 172 people attending this study were divided into two individuals of BMI < 30 and BMI? 30 according to their body mass index. The DNAs of the sample were extracted using the saturated salt method and the extracted strains from normal and obsess participants were compared with each other under electrophoresis by PCR. Results: In the group by BMI < 30 there were 57.5% male and 42.5% female participants. By contrast, in the group by BMI?30, there were 42% male and 58% female participants. The average age of the participants in BMI < 30 group was 47.55 years but in the BMI?30 group it was 52.45 years. BMI mean of the people in BMI < 30 was 25.19 and BMI mean of the people in BMI?30 was 32.58. DD genotype frequency in BMI?30 was 0.83 which is more than the frequency of BMI < 30 (0.83 against 0.71). Conclusion: The obtained results of this research suggested that genotype DD in ACE gene has a relationship with the obesity of Ardabil province population.