كليدواژه :
نشت الكتروليت , اسيدآمينه , اثرات سمي , محتواي نسبي آب
چكيده فارسي :
به منظور بررسی اثرات كاربرد گلایسین‌بتائین بر گوجه‌فرنگی در سال 1394 در مزرعه‌ای واقع در مشهد، آزمایشی دو عاملی (عامل اول مقدار محلول‌پاشی در سه سطح (صفر، سه و شش كیلوگرم در هكتار) و عامل دوم زمان محلول‌پاشی (زمان كشت، شروع گلدهی و شروع میوه‌دهی)) به‌صورت فاكتوریل در قالب طرح بلوك‌های كامل تصادفی با چهار تكرار اجرا شد. در این آزمایش محتوای نسبی آب برگ، سطح برگ، نشت الكترولیت، عدد SPAD، طول دوره رشد رویشی و زایشی، عملكرد و اجزای عملكرد گیاه گوجه‌فرنگی مورد بررسی قرار گرفت. نتابج به‌دست آمده از نشت الكترولیت‌ها و محتوای آب نسبی در كلیه تیمارها نشان داد كه گیاهان به لحاظ رطوبتی در تنش قرار نداشتند. كاربرد گلایسین‌بتائین در زمان كاشت، سبب افزایش دوره رشد رویشی و كاهش دوره رشد زایشی گیاه شد. كاربرد سه كیلوگرم در هكتار گلایسین‌بتائین در زمان كشت، منجر به افزایش سطح برگ، وزن خشك و عدد SPAD شد. بیشترین تعداد كل میوه (5/42 عدد) در تیمار كاربرد سه كیلوگرم در هكتار گلایسین‌بتائین در زمان كاشت و بیشترین وزن میوه (1/113 گرم) در تیمار شاهد به‌دست آمد. بیشترین میزان عملكرد در تیمار عدم مصرف گلایسین‌بتائین (74 تن در هكتار) مشاهده شد. سه و شش كیلوگرم در هكتار گلایسین‌بتائین، عملكرد را به‌ترتیب 15 و 30 درصد كاهش داد، اما با توجه به افزایش طوره دوره رشد رویشی و افزایش تعداد میوه‌های سبز در تیمار سه كیلوگرم در هكتار، احتمالاً مصرف گلایسین‌بتائین در دوزهای پایین در زمانی تأثیر مثبت خواهد داشت كه طول دوره رشدی محدودكننده نباشد. همچنین به نظر می‌رسد كاربرد دوزهای بالای گلایسین‌بتائین در شرایطی كه تنش جدی گیاه را تهدید نمی‌كند، سبب بروز اثرات سمی و كاهش عملكرد محصول گوجه‌فرنگی گردید.
چكيده لاتين :
Introduction
Glycine Betaine has potential impacts on enhancing the tolerance of plants against various environmental abiotic stresses. There are two methods of increasing Glycine Betaine level in plants in which Glycine Betaine does not accumulate naturally, the exogenous application of Glycine Betaine and the introduction of GB-biosynthetic pathway into plants, via transgenes. When Glycine Betaine is applied to the leaves of tomato plants, most of the Glycine Betaine that is taken up by the leaves is localized in the cytosol which is significantly reduced adverse effects of abiotic stresses (Park et al., 2006). Even in non-stress conditions, there are several environmental factors (water scarcity between irrigation times, soil salinity, mid-day heat, etc.) that can affect the plant yield. This study tried to find the effectiveness of exogenous application of Glycine Betaine on tomato plant in non-stress condition.
Materials and Methods
The experimental was conducted in 2015 as the randomized complete block design based on factorial with four replications. Treatments included three levels of Glycine Betaine application level (0, 3, 6 kg.ha-1) and three times of application including planting, floral initiation and fruit set. 0, 3 and 6 kgha-1 Glycine Betaine applied with 200 lit.ha-1 water and applied with back sprayer. The experimental field was located 10 km southeast of Mashhad. Irrigation with 6-day interval was done to represent non-stress condition. Electrolyte leakage, SPAD number and Relative Water Content (RWC) were measured. Dry matter, leaf area, days from planting to flowering, days from flowering to fruit set, total fruit numbers, ripe fruit numbers, green fruit numbers, average fruit size, and total yield were determined during the study. Harvest time was determined by meteorological forecast and before the first freezing stress in fall. The data were analyzed by SAS software and the means were compared by Duncan.
Results and Discussion
Results showed that the times of application had significant effects on some measured parameters. Glycine Betaine application at planting stage led to increasing vegetative stage and delayed flowering time for 6 days. The highest leaf area (1464.4 cm2) was also obtained from the same application. Different times of Glycine Betaine application had no significant effects on total yield. However, using at planting time resulted in higher green/total fruit ratio. Evaluation the rate of Glycine Betaine application showed that the highest leaf area (1370 cm2) and the highest dry matter (1206 g.ha-1) were obtained from 3 kg.ha-1 application rate. Treatments without GB application resulted in the highest yield (74.7 ton. ha-1), but increasing the rate from 0 to 3 kg.ha-1, led to 94% increasing in green fruit numbers (8.4 to 16.2 respectively).
Conclusions
Exogenous application of Glycine Betaine showed different effects on tomato plants due to time and rate of application. The highest yield was obtained from control treatment (no application) (74 ton ha-1) and 3 and 6 kg.ha-1 applications, decreased yield 15 and 30 percent, respectively. However, the vegetative stage was lengthened and the numbers of green fruits was increased by 3 kg.ha-1 rate. It seems that if the growing season was extended, and enough time was provided to fruit ripening, total yield could be increased by low concentration of Glysine Betaine application up to 25% compared to control. But high rate of Glycine Betaine may cause toxic effects on tomato and reduce the yield.
