اثر سميت ليگاند‌هاي بازشيف مشتق شده از معرف گيرارد-T و كمپلكس‌هاي دي‌متيل قلع(IV) آن‌ها بر سلول‌هاي سرطاني كلون

Cytotoxic Effect of Girard-T Reagent-based Schiff Bases and their Dimethyltin (IV) Complexes on Human Colon Cancer Cells

علي رغم تلاش هاي فراوان هنوز درمان قطعي براي سرطان شناخته نشده است. بنابراين مطالعه تركيباتي با خواص ضد سرطاني اهميت ويژه اي دارند. هدف اصلي اين پژوهش بررسي سميت ليگاند‌هاي بازشيف معرف گيرارد-T و كمپلكس‌هاي قلع(IV) آنها بر سلول هاي سرطاني كولون مي باشد. سلول هاي HT29 در محيط DMEM/F12 حاوي 10% FBS و آنتي بيوتيك 1% كشت شدند و تاثير ليگاندها و كمپلكس هاي آنها باغلظت هاي 0/1، 1 و 5 mg/ml براين سلول ها در روزهاي 1و 2 و 3 بررسي شد. ميزان رشد، تكثير و تغييرات مورفولوژيك ايجاد شده با ميكروسكوپ معكوس، بررسي شد و ميزان بقاي سلول ها با آزمون MTT و رنگ آميزي DAPI تعيين گشت. تيمار سلول هابا تركيبات بازشيف منجر به القاي مرگ سلولي بصورت وابسته به غلظت گرديد بطوري كه بعد از 72 ساعت بقاي سلول هاي تيمار شده با غلظت5 (موثرترين غلظت) mg/ml با (H2L1) ،(H2L2) ،[SnMe2(L1)]،[SnMe2(L2)] به ترتيب 22، 29، 18 و 22 بود (0/05

Despite many efforts, no definitive treatment is yet knowing for cancer. Therefore, research on new compounds that have anti-cancer properties is especially important. The aim of this study is investigation the cytotoxic effect of Girard-T reagent-based Schiff bases and their complexes on HT29 cancer cells. HT29 cells was cultured in DMEM/F12 medium containing FBS 10% and antibiotics 1% then the effects of ligands and their complexes with the concentration of 0.1, 1 and 5 mg/ml were surveyed on these cells in 1, 2, and 3 days. Growth, proliferation and morphological changes were photographed using an inverted microscope. MTT assay and DAPI staining were used to quantify cell viability. With concentrations of 0.1, 1, and 5 mg/ml of [SnMe2(L2)] (the effective component) the survival were decreased to 34, 24 and 18% after 72h(P<0.05). Encountering cells with complex caused to induce apoptosis in relation with concentration so that, the cell viability of cells treated with concentrations of 5 mg/ml (the effective concentration) of (H2L1) ،(H2L2) ،[SnMe2(L1)]،[SnMe2(L2)] was 22, 18, 29, and 22 respectivly after 72h and the highest apoptosis (82%) happened in 5mg/ml of SnMe2(L2). The synthetic components have a toxic and ant-proliferation effect on HT29 in relation with concentration