بررسي اثرات تركيب اسپيناستين-7-O- بتا گلوكوزيد بر مهار رشد و القاي آپوپتوز در رده سلولي MCF-7 سرطان پستان

Evaluation of spinacetin 7-O-β-D-glucopyranoside in the induction of apoptosis in breast cancer cell line MCF-7

زمينه و هدف: اين مطالعه به‌منظور بررسي تأثير اسپيناستين گليكوزايد تركيب فلاونوئيدي جداشده از گياه Centurea shmidi بر مهار رشد و القاي آپوپتوز در رده سلولي MCF-7 سرطان پستان انجام شد. روش بررسي: در اين مطالعه ميزان مهار رشد سلولي و القاي آپوپتوز در سلول‌هاي حاصل از كشت رده‌هاي سلولي MCF-7 پس از تيمار با غلظت‌هاي مختلف اسپيناستين گليكوزايد به‌وسيله‌ي روش‌هاي MTT و فلوسايتومتري مورد بررسي قرار گرفت. يافته‌ها: بر طبق نتايج حاصل از آزمون MTT بعد از تيمار 48 ساعته با تركيب اسپيناستين-7-O-بتا گلوكوزيد در قياس با گروه كنترل مهار معني‌دار تكثير سلولي مشاهده گرديد(0/05

Evaluation of spinacetin 7-O-β-D-glucopyranoside in the induction of apoptosis in breast cancer cell line MCF-7 Aghaei M1, Ghanadian SM2*, Fallah M2, Solimani Dehkordi E3 1Clinical Biochemistry Dept., Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; 2Pharmacognosy Dept., Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran; 3Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran. Received: 4/Jul/2017 Accepted: 8/Nov/2017 Background and aims: This study was aimed to evaluate the effect of spinacetin 7-O-β-D-glucopyranoside, a flanovid compound separated from Centurea shmidi against MCF-7 breast cancer cell line and its effect on apoptosis induction. Methods: In this study, the inhibition rate of cell growth and induction of apoptosis in MCF-7 cell culture cells after treatment with different concentrations of spinostine glycoside was studied by MTT and flow cytometry. Results: According to the results of MTT test, after 48 hours treatment with spinostin-7-O- β-D-glucoside, a significant inhibition in cell proliferation was observed compared to the control group (P <0.05). The results of the study of apoptosis in the flow cytometry assay showed that the derived flavonoid increased significantly the apoptosis in MCF-7 breast cancer cell line (P<0.05). Conclusion: The derived flavonoid of spinacetin-7-O-β-D-glucopyranoside significantly inhibited MCF-7 proliferation and induced apoptosis. So, it could be suggested as a compound for breast cancer therapy.