كليدواژه :
مونوساكاريد دي ساكاريد , نانو ذرات طلا , تست هاي جريان جانبي , زيست حسگر
چكيده فارسي :
زمينه و هدف: در سال هاي اخير علاوه بر روش هاي مولكولي متعدد موجود جهت تشخيص، روش هاي مبتني بر طراحي انواع زيست حسگرها (Biosensors) مورد توجه قرار گرفته است. نوعي از اين زيست حسگرها
تست هاي جريان جانبي (Lateral Flow Test) مبتني بر نانو ذرات طلا مي باشند. با توجه به اهميت كاربرد نانو ذرات طلا به‌عنوان نشانگر در زيست حسگرها و نيز لزوم استفاده از قندها در ساخت زيست حسگرها، هدف از اين پژوهش بررسي تأثير انواعي از قندها (مونوساكاريدها و دي ساكاريدها) بر نانو ذرات طلا جهت ساخت زيست حسگر تشخيصي مي باشد.
روش بررسي: در مطالعه آزمايشگاهي حاضر، پس از سنتز نانو ذرات طلا به‌صورت شيميايي، سايز، بيشينه جذب در 520 نانومتر و غلظت نانو ذرات با استفاده از اسپكتروفتومتر و روش DLS مورد ارزيابي قرار گرفت. سپس با توجه به اهميت استفاده از قندها در ساخت زيست- حسگرها، شيب غلظتي 5 تا 500 ميلي مولار از قندهاي گلوكز، فروكتوز، ساكاروز و ترهالوز تهيه و طيف جذبي آن‌ها، بعد از تأثير بر نانو ذرات طلا در طول موج 450 تا 700 نانومتر به دست آمد.
يافته‌ها: بررسي نتايج حاصل از خوانش طيف اسپكتروفتومتري نانو ذرات سنتز شده، در حضور و عدم حضور انواع قندهاي مونوساكاريد و دي ساكاريد ذكر شده، نشان دهنده كاهش بيشينه جذب نانو ذرات پس از اعمال شيب غلظتي انواع قند بر آن ها مي باشد و همچنين شيب غلظتي اعمال شده از قندهاي گلوكز، ساكارز و ترهالوز تأثيري بر تجمع و رسوب نانو ذرات طلا نداشته؛ در حالي كه فروكتوز در غلظت هاي 50 ميلي مولار به بالا باعث تجمع نانو ذرات طلا مي‌شود.
نتيجه‌گيري: به دليل ضرورت استفاده از قندها در ساخت نانو حسگرهاي زيستي، به نظر مي‌رسد استفاده از قندهاي كتوني ازجمله فروكتوز در غلظت هاي 50 ميلي مولار به بالا جهت ساخت زيست حسگر مناسب نمي‌باشد.
چكيده لاتين :
Background and Aims: Recently, in addition to numerous molecular methods available for diagnosis, methods based on the design of various biosensors are widely used. Some of these biosensors are Lateral flow tests based on gold nanoparticles. Considering the importance of the application of gold nanoparticles as markers in biosensors and also the need for the use of sugars in the construction of biosensors, this study was proposed to investigate the effects of various sugars (monosaccharides and disaccharides) on gold nanoparticles for the development of diagnostic biosensors.
Methods: In this experimental study, after synthesis of gold nanoparticles chemically, by size, measuring maximum absorption at 520 nm, and concentration of nanoparticles a spectrophotometer and DLS method were used. Then, considering the importance of using sugars in the manufacture of biosensors, a gradient of Background and aims: Recently, in addition to numerous molecular methods available for diagnosis, methods based on the design of various biosensors considered. Some of these biosensors are Lateral flow tests based on gold nanoparticles. Considering the importance of the application of gold nanoparticles as markers in biosensors and also the need for the use of sugars in the construction of biosensors, this study was aimed to investigate the effects of various sugars (monosaccharides and disaccharides) on gold nanoparticles for the development of diagnostic biosensors.
Methods: In this experimental study, after synthesis of gold nanoparticles chemically, by size, assessed maximum absorption at 520 nm, and concentration of nanoparticles by spectrophotometer and DLS method. Then, considering the importance of using sugars in the manufacture of biosensors, a concentration gradient of 5 to 500 mM glucose, fructose, sucrose, and trehalose, and their absorption spectra, after exposure to gold nanoparticles at 450 to 700 nm was detected.
Results: The results of the spectrophotometric spectrum of synthesized nanoparticles in the presence and absence of sugars of monosaccharide and disaccharide indicate a decrease in the maximum absorption of nanoparticles after the application of a steady slope of sugar types on them and also concentration gradient of glucose, sucrose and trehalose sugars had no effect on the accumulation and aggregation of gold nanoparticles, while fructose at concentrations up to 50 mM leads to the accumulation of gold nanoparticles.
Conclusion: Due to the necessity of using sugars in the production of bionanosensors, it seems that the use of ketone sugars, such as fructose, at concentrations of 50 mM upwards is not suitable for making biosensors.
5 to 500 mM glucose, fructose, sucrose, and trehalose, and their absorption spectra, after exposure to gold nanoparticles at 450 to 700 nm were utilized.
Results: The results of the spectrophotometric spectrum of synthesized nanoparticles in the presence and absence of various sugars of monosaccharide and disaccharide, indicates a decrease in the maximum absorption of nanoparticles after the application of a steady slope of sugar types on them. Slope of glucose, sucrose and trehalose sugars has no effect on the accumulation and aggregation of gold nanoparticles, while fructose at concentrations up to 50 mM leads to the accumulation of gold nanoparticles.
Conclusion: Due to the necessity of using sugars in the production of bionanosensors, it seems that the use of ketone sugars, such as fructose, at concentrations of 50 mM upwards is not suitable for making biosensors.
